Wako Pure Chemical Industries, Ltd.Wako Pure Chemical Industries, Ltd. - Laboratory Chemicals

Precast gel with a clear band
SuperSep Ace

Super Sep Ace is a polyacrylamide precast gel for electrophoresis which was achieved a high separability and long-term storage stability. Not only the general SDS-PAGE, but also the electrophoresis of DNA is possible. Further, because any SDS is not contained in the gel, it can also be used for Native-PAGE.


1. Features

Feature 1: Good separation and a clear band


Feature 2: Compatible with multi-channel pipette



Feature 3: Attached with a sticker to understand the types of gel easily on the side face of the box


           

2. Product Specifications

Plate size
100 x 100 x 3 (mm)
Gel size
90 x 85 x 1 (mm)
Well number
13
17
Well volume (μL)
30
25

3. Migration Buffer

Protein Sample Buffer ( x 2 ) 0.125M Tris-HCl, pH6.8、20% Glycerol、4% SDS、10%
2-Mercaptoethanol, 0.004% BPB (Wako Catalog No.196-11022)
Migration Buffer ( x10 ) 0.25M Tris, 1.92M Glycine, 1% SDS (Wako Catalog No.184-01291)
Low-molecular-weight protein Sample Buffer ( x 2 ) 0.125M Tris-HCl, pH6.8, 20% Glycerol, 4% SDS, 10%
2-Mercaptoethanol, 0.004% BPB (Wako Catalog No.196-11022)
Migration Buffer ( x10 ) 0.5M Tris, 0.5M Tricine, 1% SDS (Wako Catalog No.200-17071)
Nucleic Acid Sample Buffer ( x 2 ) 0.01M Tris-HCl, pH7.9, 1mM EDTA, 30% Sucrose, 0.004%
BPB or Loading Buffer (Wako Catalog No.313-90111)
Migration Buffer ( x10 ) 0.25M Tris, 1.92M Glycine or TBE buffer (Wako Catalog No.318-90041)

4. Migration patterns by concentration

Please select the concentration of acrylamide referring to the following migration patterns.


WIDE-VIEW™ Prestained Protein Size Marker III is used.

5. Product List

Product Name The number of well Package Size Wako Catalog No. Storage Condition
SuperSep Ace, 6% 13 10 EA 195-15171 Keep at 2-10°C.
SuperSep Ace, 7.5% 13 10 EA 198-14941
17 10 EA 191-14931
SuperSep Ace, 10% 13 10 EA 195-14951
17 10 EA 192-14961
SuperSep Ace, 12.5% 13 10 EA 199-14971
17 10 EA 196-14981
SuperSep Ace, 15% 13 10 EA 193-14991
17 10 EA 190-15001
SuperSep Ace, 5-12% 13 10 EA 199-15191
17 10 EA 192-15201
SuperSep Ace, 5-20% 13 10 EA 197-15011
17 10 EA 194-15021
SuperSep Ace, 10-20% 13 10 EA 191-15031
17 10 EA 198-15041
SuperSep Ace, 15-20% (Tricine Gel) 13 10 EA 198-15301
17 10 EA 198-17361

6. Premix Buffer

Product Name Description Pkg. Size Wako Cat. No. Storage Condition
Sample Buffer Solution (2ME+)( x 2) Sample buffer for the Laemmli method, containing Mercaptoethanol 25 mL 196-11022 Keep at 2-10°C.
Sample Buffer Solution (2ME+)( x 4) 25 mL 191-13272
Sample Buffer Solution
(2ME-)( x 2)
Sample buffer for the Laemmli method, without Mercaptoethanol 25 mL 193-11032
Sample Buffer Solution
(2ME-)( x 4)
25 mL 198-13282
Running Buffer Solution
( x 10)
Tris-glycine Migration Buffer for the Laemmli method, containing SDS 1 L 184-01291
Tricine Running Buffer Soln. (2ME-)( x 10) Migration Buffer for Tris-Tricine Gel 1 L 200-17071
Loading Buffer
[Nippon Gene Co., Ltd.]
Loading Buffer for nucleic acid sample 10 mL 313-90111 Keep at RT.
5 x TBE
[Nippon Gene Co., Ltd.]
Migration Buffer for nucleic acid sample 1 L 318-90041

RT: room temperature

7. EasySeparator

Features

  • You can set the gel simply by rotating the knob.
  • Migration can be performed only with a small amount of migration buffer (more than about 350 mL).

EasySeparator




YouTube" and "YouTube logo" are trademark or registered trademark of Google Inc..

"The video of how to use is open to public on YouTube. (Language: Japanese)

You can see the procedure at YouTube

Product Name Package Size Wako Catalog No.
EasySeparator 1 set 058-07681

8. Related Products

SuperSep Phos-tag™

A precast gel copolymerized with Phos-tag™ acrylamide. It requires neither phospho-specific antibody nor radioactive isotope labeling.