Wako Catalog No. 317-07123 (30 units); 311-07121 (300 units)
10 minute-Simple protocol
Hydrolyzing gel solution is directly available for cloning, restriction endonuclease digestion, sequencing, etc.
Attains extraction of intact large DNA from agarose gels.
β-agarase is an enzyme which hydrolyzes β-1,4 linkages in agarose to produce neoagaro- oligosaccharides. Agarose digested by β-agarase does not gelate again, therefore, nucleic acids can be recovered from agarose gels. Thermostable β-Agarase has a higher thermostability and stronger hydrolyzing activity than conventional β-agarase. Furthermore, a simple protocol allows for quick DNA and RNA purification. This enzyme is particularly suitable for purification of intact large DNA.
This enzyme was isolated from deep-sea microorganism by Japan Agency for Marine-Earth Science and Technology (JAMSTEC) using the submergible SHINKAI6500.
10 minute Protocol
Transfer the 200 mg gel slice to a 1.5 mL microcentrifuge tube.
Add 6 units of the Thermostable β-Agarase.
Incubate at 65°C for 10 minutes. During incubation, tap the tube every 3~5 minutes.
Chill on ice for 5 minutes, and make sure that agarose does not gelate again.