Genetic Research

for Recovery of nucleic acids from agarose gels

Thermostable β-Agarase

Wako Catalog No. 317-07123  (30 units);  311-07121  (300 units)

Features

10 minute-Simple protocol

Hydrolyzing gel solution is directly available for cloning, restriction endonuclease digestion, sequencing, etc.

Attains extraction of intact large DNA from agarose gels.

β-agarase is…

β-agarase is an enzyme which hydrolyzes β-1,4 linkages in agarose to produce neoagaro- oligosaccharides. Agarose digested by β-agarase does not gelate again, therefore, nucleic acids can be recovered from agarose gels. Thermostable β-Agarase has a higher thermostability and stronger hydrolyzing activity than conventional β-agarase. Furthermore, a simple protocol allows for quick DNA and RNA purification. This enzyme is particularly suitable for purification of intact large DNA.

This enzyme was isolated from deep-sea microorganism by Japan Agency for Marine-Earth Science and Technology (JAMSTEC) using the submergible SHINKAI6500.

SHINKAI 6500

Procedure

10 minute Protocol

Transfer the 200 mg gel slice to a 1.5 mL microcentrifuge tube.

Add 6 units of the Thermostable β-Agarase.

Incubate at 65°C for 10 minutes. During incubation, tap the tube every 3~5 minutes.

Chill on ice for 5 minutes, and make sure that agarose does not gelate again.