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for Cleavage of His tag & GST tag fusion proteins
Turbo3C Protease, recombinant, Solution
[ HRV3C Protease ]

HRV3C Protease (Human rhinovirus 3C Protease) is a cysteine protease which recognizes the cleavage site; Leu-Glu-Val-Leu-Phe-Gln Gly-Pro.  HRV3C Protease is HRV3C Protease with His tag and GST tag. Therefore Turbo3C Protease can be removed by either Ni chelating resin or GSH (Glutathione) resin.   Turbo3C Protease has significant activity at 4°C, so it is difficult for other proteases to show activity and the target protein may be obtained with the stable form.

This product is not available for sales in the US and China.



  INDEX
  1. Product Overview
  2. Data
  3. Product List
  4. Links:  

1. Product Overview
  • Source: E. coli expressed Turbo3C protease (human rhinovirus 3C protease)
  • Storage Buffer: 25 mmol/L Tris-HCl (pH 8.0), 50 mmol/L NaCl, 1 mmol/L TCEP-HCl and 50% Glycerol
  • Activity: Indicated on each label
  • Unit Definition: 1 unit is the amount of enzyme which cleaves 95+ % of 100 μg of target protein at 4°C for 16 hours.
  • Protein Concentration: Indicated on each label.
  • Molecular Weight: approx. 47,000
  • Cleavage Site: Leu-Glu-Val-Leu-Phe-Gln Gly-Pro
    (LEVLFQ GP)

2. Data

M: Molecular Weight Marker
Lane 1: Tag fusion protein
Lane 2: Tag fusion protein + Turbo3C Protease


As a result of reaction of 50 μg of GST fusion protein (68 kDa), which has HRV3C Protease recognition sequence with 0.5 units of Turbo3C Proease at 4°C for 16 hours, the protein was cleaved into two fragments: 42 kDa and 26 kDa.
3. Product List
Product Name Grade Pkg. Size Wako Cat. No. Storage Condition
Turbo3C Protease, recombinant, Solution
[ HRV3C Protease ]
for Genetic Research 1 mg 206-18151 Keep at -20°C
This product is not available for sales in the US and China.

4. Link
⇒ Links to Leaflet (496 KB/2 P)

Leaflet
⇒ Links to Package Insert (264 KB)

Package Insert


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