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Home > Products > Life Science > Protein Research  >  WESTERN-VIEW™ Western Protein Size Marker (32 - 165 kDa)

Protein Molecular Marker for Western Blot
WESTERN-VIEW™ Western Protein Size Marker (32 - 165 kDa)


This is a protein molecular marker for Western blotting containing 5 types of antibody-binding bands (32 - 165 kDa) and 2 types of chromatophore-binding bands (blue: 29 kDa, orange: 105 kDa). The 5 types of antibody-binding proteins will bind to antibodies in use and appear as bands upon detection, which makes any additional procedure or reagent unnecessary.
Each protein is an E. coli-expressing recombinant protein.


1. Features


  • Detectable with Western blotting
  • 2 types of chromatophore-binding bands and 5 types of antibody-binding bands
  • No boiling/dilution required

2. Usages


  1. Bring the product back to room temperature before use.
  2. Mix the solution uniformly using pipette or vortex.
  3. Apply 2 - 5 μL/lane to a gel.
  4. After being transferred to a membrane, an antibody in use will also bind to the marker during antibody reaction.
Gel: SuperSep™ Ace, 10-20%, 17 wells (Wako Cat. No.198-15041 )
Running Buffer: SDS-PAGE Buffer, pH 8.5 (Wako Cat. No.192-16801 )
Protein Molecular Marker: This product (Wako Cat. No.236-02723 ) 5μL/lane
Membrane: Clear Trans SP PVDF Membrane, Hydrophobic, 0.2 μm (Wako Cat. No. 033-22453 )
Antibody: Anti Goat IgG, Peroxidase conjugated
Chemiluminescent reagent: ImmunoStar Zeta (Wako Cat. No.295-72404

3. Composition


125 mmol/L Tris-HCl (pH 6.8), 10 mmol/L DTT, 5 mmol/L EDTA,
17.4 w/v% glycerol, 3 w/v% SDS, 0.01 w/v% phenol red.

[NOTE]
  • Avoid repetitive freezing and thawing
  • Detection sensitivity of the product largely depends on an antibody and detection reagent in use.
  • Detection system with high sensitivity may result in the appearance of extra bands. Please reduce the loading amount per lane.
  • The product is not compatible with Native-PAGE.

Standard curve

Standard curve of the antibody-binding bands (32-165 kDa)

4. Product List


Product Name
Package Size
Wako Cat. No.
Grade
Storage Condition
WESTERN-VIEW™ Western Protein Size Marker
(32 - 165 kDa)
25 μL
for Electrophoresis
Keep at -20°C
250 μL

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5. Related Products


Product Name
Package Size
Wako Cat. No.
Grade
Storage Condition
SuperSep™ Ace, 10-20%, 17 well
10 each
for Electrophoresis
Keep at 2-10°C.
SDS-PAGE Buffer, pH 8.5
5 L
Keep below 25°C.
ClearTrans SP PVDF Membrane, Hydrophobic
0.2 μm
for Blotting
Keep at room temperature.
ImmunoStar Zeta
200 cm2
Keep at -80°C.
2000 cm2
1000 cm2


Home > Products > Life Science > Protein Research  >  WESTERN-VIEW™ Western Protein Size Marker (32 - 165 kDa)