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Enhancer of post-cryopreservation survival and cloning efficiency of human embryonic stem cells and human iPS cells
Y-27632

Y-27632 is a highly potent, cell-permeable and selective inhibitor of Rho-associated coiled coil forming protein kinases (ROCK). As well as modulating smooth-muscle contraction by inhibiting Ca2+ sensitization, Rho/ROCK pathway may help to regulate integrin-mediated cell adhesion and motility, which could be critical in processes such as tumour-cell metastasisand immunoactivation. (Ki value of Y-27632 for p160ROCK = 140 nmol/L 6))
Sasai et al. reported in 2007 that it could inhibit cell death due to dispersion when it is added in human ES cell culture and it has attracted attention on various kinds of the effect.
Recently, it has been reported that when this product is added in the cryopreserving solution or in the culture medium after thawing the solution of human ES cells or human iPS cells to be 10 μmol/L, the colony forming activity greatly improves. Also, when cryopreserved ES cells were thawed and cultured in the medium including this product, the colony forming activity was confirmed to be improved.


1. Link

2. Features

  • High quality: Assay: 98.0+ % (HPLC)
  • Low cost
                Y-27632

3. Protocol

①hESCs maintenance culture



②Dissociated hESCs splitting in the presence of 10μmol/L Y-27632



③Slow freezing of single hESCs in the presence of 10μmol/L Y-27632



④Thawed rapidly in 37°C bath



⑤Seeded in hESCs maintenance medium in the presence of Y-27632


4. Product List

Product Name Pkg. Size Wako Cat. No. Grade Storage Condition
Y-27632 1 mg 257-00511 for Cellbiology
Keep at -20°C.
5 mg 253-00513
25 mg 251-00514
Y-27632, MF 5 mg 259-00613 for Cell Culture
25 mg 257-00614
CultureSure Y-27632 1 mg 030-24021
5 mg 036-24023
25 mg 034-24024
CultureSure 10 mmol/L Y-27632 Solution,
Animal-derived-free
300 μL 039-24591
5 mmol/L Y-27632 Solution 300 μL 253-00591
These products are marketed under the license of Mitubishi Tanabe Pharma Corporation.


5. Related Products

Product Name Pkg. Size Wako Cat. No. Grade Storage Condition
CultureSure A-83-01 2 mg 039-24111 for Cell Culture Keep at -20°C
10 mg 035-24113
CultureSure CHIR99021 1 mg 038-23101
5 mg 034-23103
CultureSure CKI-7 Dihydrochloride 5 mg 035-23971
CultureSure IWP-2 5 mg 034-24301
25 mg 030-24303
CultureSure SB431542 5 mg 031-24291
25 mg 037-24293

6. References

  1. Uehata, M. et al.: Nature, 389, 990 (1997) 
  2. Sakamoto, K. et al.: J. Pharmacol. Sci., 92, 56 (2003)
  3. Nishimaru, K. et al.: J. Pharmacol. Sci., 92, 424 (2003)
  4. Sasai, Y. et al.: Nat. Biotechnol., 25, 681 (2007)
  5. R. Martin-Ibanez. et al.: Hum. Reprod, 23, 2744 (2008)
  6. Classen A. D. et al.: Mol. Reprod. Dev., 76, 722 (2009)
Home > Products > Life Science > Y-27632