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Endotoxin Detection Reagents Limulus ES-Ⅱ Series

  • Turbidimetric Technique
  • Gel-clot Technique
  • Endotoxin Specific

Features

  • Highly sensitive endotoxin specific detection without the interference of (1 → 3)-β-D-glucan in the sample is possible.
  • Kit and reagent compatible with the Bacterial Endotoxins Test specified in BET (JP)
  • The LAL reagent is labeled with gelation sensitivity in titers (EU/mL) measured using Reference Control Endotoxin of JP.
  • Firm gel formation capability to facilitate determination of gelation
  • A multi-test type requires the dissolution of the reagent in water for the endotoxin test, while a single-test type only requires the addition of 0.2 mL of sample.
  • Highly sensitive analysis is available using a Toxinometer®.
  • Turbidimetric Technique with Toxinometer® can detect up to 0.0078 - 1 EU/mL.
  • Stable endotoxin measurement is possible, due to the minor pH impact of the measurement sample on testing.
  • Testing on water and various drug products is possible and it is ideal for endotoxin detection in animal blood or bovine milk.
Single test Type
Code No. Product Name Labeled Gelation Sensitivity (EU/mL) Quantity
295-51301
with CSE
Limulus ES-Ⅱ Single Test Wako 0.015 25 tests

Kit: Limulus ES-Ⅱreagent, 1 sample for 0.2 mL x 25 vials; Control Standard Endotoxin (CSE) x 1 vial

Multi test Type
Code No. Product Name Labeled Gelation Sensitivity (EU/mL) Quantity
299-51201
with CSE
Limulus ES-Ⅱ Test Wako 0.015 60 tests

Kit: Limulus ES-Ⅱ reagent for 2 mL x 3 vials; Control Standard Endotoxin (CSE) x 1 vial
LAL reagent solution can be stored below −20 ̊℃ for up to 2 weeks.

Multi-type Single Product
Code No. Product Name Labeled Gelation Sensitivity (EU/mL) Quantity
292-51213
Limulus Amebocyte Lysate ES-Ⅱ,
Lyophilized
0.015 100 tests

Kit: ES-Ⅱ reagent for 2 mL x 5 vials

The Control Standard Endotoxin mentioned in the kits above is a lyophilized product, purified from E. coli UKT-B, containing 500 ng endotoxin, as well as mannitol and glycine as additives in each vial. Each lot is labeled with a typical value (EU/vial) measured by using Reference Control Endotoxin of JP.

Experiment Example

The measurement is performed for 200 minutes and analyzed via a highly sensitive analysis method using a Toxinometer® and Limulus ES-Ⅱ Single Test Wako. The standard curve obtained shows a high correlation within a wide concentration range from 0.125 to 0.0078 EU/mL.

Time Course Data

Topics

  • LAL Basic Knowledge
  • Lysate reagent selection flowchart

Contact

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