試薬ホーム > HPLCアプリケーションデータ > Analysis of Acids

HPLCアプリケーションデータ
Analysis of Acids

Next page

• Analysis of Amino acid of Bean Hydrolysate
• Analysis of Amino acid of Strawberry
• Analysis of Amino acid of Tea (include Theanine, gamma-aminobutyric Acid) and Vitamin C
• Analysis of Amyloid β-protein (1-40)
• Analysis of Arginine , Taurine and Aspartic Acid
• Analysis of Aromatic Organic Acids
• Analysis of Linear Chained Carboxylic Acid
• Analysis of MW marker
• Analysis of MW marker
• Analysis of NBD-F-Tyrosine

Analysis of Amino acid of Bean Hydrolysate (PDF file=300KB)

Column	Wakosil -PTC, 4.0mm I.D.×200mm
Eluent	A）PTCアミノ酸溶離液A B ）PTCアミノ酸溶離液B 0-15min. B conc. 0-70%, 15-20min. B conc. 100%, 20-30min. B conc. 　0%
Flow rate	1.0ml/min. at 40℃
Detector	UV 254nm 0.064AUFS
Injection	10μl
Sample	Asp Glu Ser Gly His Arg Thr Ala Pro Tyr Val Met Cys Ile Leu Phe Lys
Category	Acid

Analysis of Amino acid of Strawberry　 (PDF file=278KB)

Column	Wakosil -PTC, 4.0mm I.D.×200mm
Eluent	A）PTCアミノ酸溶離液A B ）PTCアミノ酸溶離液B 0- 2min. B conc. 0%, 2-17min. B conc. 0-70%, 17-20min. B conc. 100%. 20-27min. B conc. 0%
Flow rate	1.0ml/min. at 40℃
Detector	UV 254nm 0.064AUFS
Injection	10μl
Sample	Asp Glu Gly Arg Ala Thr Pro Val Met
Category	Acid

Analysis of Amino acid of Tea (include Theanine, gamma-aminobutyric Acid) and Vitamin C　 (PDF file=354KB)

Column	Wakosil -PTC, 4.0mm I.D.×200mm
Eluent	A）PTCアミノ酸溶離液A B ）PTCアミノ酸溶離液B 0- 2min. B conc. 0%, 2-17min. B conc. 0-70%, 17-20min. B conc. 100%, 20-25min. B conc. 0%
Flow rate	1.0ml/min. at 40℃
Detector	UV 254nm
Injection	50μl
Sample	Asp Glu Ser Gly His Arg Theanine Leu
Category	Acid

Analysis of Amyloid β-protein (1-40)　 (PDF file=50KB)

Column	Wakosil-II5C18 AR, 4.6mm I.D. x 150mm
Eluent	A) H2O 0.1% TFA B) CH3CN 0.1% TFA 0-25min. B conc. 20-70%, 25-30min. B conc. 70%
Flow rate	1.0mL/min, at 40C
Detector	UV 220nm, 0.512AUFS.
Injection	2uL, 2mg/mL
Sample	Amyloid β-protein (1-40)
Category	Proteins, Peptides, Amino Acids

Analysis of Aromatic Organic Acids　 (PDF file=49KB)

Column	Navi C18-5, 4.6mm I.D. x 250mm
Eluent	CH3OH/50mM NaH2PO4 (pH=2.5, by 50mM H3PO4)=40/60(v/v)
Flow rate	1.0mL/min, at 35C
Detector	UV 210nm, 0.16AUFS.
Injection	10uL
Sample	1,2,4,5-Benzenetetracarboxyic Acid (5ug/mL) Phthalic Acid (5ug/mL) Trimesic Acid (5mg/mL) Benzoic Acid (50ug/mL) in Eluent
Category	Acids

Analysis of Linear Chained Carboxylic Acid　 (PDF file=304KB)

Column	Navi C22-5, 4.6mm I.D.×250mm
Eluent	20mM NaH2PO4（pH2.5）
Flow rate	1.0ml/min. at 35℃
Detector	UV 210nm, 0.04 AUFS
Injection	10μl
Sample	Oxalic Acid Tartaric Acid Formic Acid Lactic Acid Acetic Acid Citric Acid Fumaric Acid Succinic Acid Acrylic Acid Propionic Acid Levulinic Acid
Category	Acid

Analysis of MW marker　 (PDF file=49KB)

Column	Wakosil 5Diol-200, 8.0mm x 300mm
Eluent	0.1M NaH2PO4 (pH7.0) in 0.2M NaCl
Flow rate	1.0ml/min, at 40C
Detector	UV 210nm, 0.32AUFS.
Injection	5ul
Sample	Glutamate Dehydrogenase (MW 290,000) Lactate Dehydrogenase (MW 142,000) Enolase (MW 67,000) Adenylate Kinase (MW 32,000) Cytochrome C (MW 12,400)
Category	Proteins, Peptides, Amino Acids

Analysis of MW marker　 (PDF file=50KB)

Column	Wakosil 5Diol-200, 8.0mm x 300mm
Eluent	0.1M NaH2PO4 (pH7.0) in 0.2M NaCl
Flow rate	1.0ml/min, at 40C
Detector	UV 280nm, 0.002AUFS.
Injection	5ul
Sample	Glutamate Dehydrogenase (MW 290,000) Lactate Dehydrogenase (MW 142,000) Enolase (MW 67,000) Adenylate Kinase (MW 32,000) Cytochrome C (MW 12,400)
Category	Proteins, Peptides, Amino Acids

Analysis of NBD-F-Tyrosine　 (PDF file=50KB)

Column	Wakosil-II5C18 HG, 4.6mm I.D. x 250mm
Eluent	A) 5mM NaH2PO4, Na2HPO4(pH6.8) B) CH3CN 0-30min. B conc. 25-35%, 30-35min. B conc. 35%, 35-40min. B conc. 100%
Flow rate	1.0mL/min, at 40C
Detector	Ex 488nm Em 540nm RANGE＝1, SENSE＝H, ATTEN＝1
Injection	20uL
Sample	NBD-F- 1. o-Tyrosine 2. m-Tyrosine 3 p-Tyrosine
Category	Proteins, Peptides, Amino Acids