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HPLCアプリケーションデータ
Analysis of Acids

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Analysis of Organic Acids (PDF file=50KB)

Column Navi C22-5, 4.6mm I.D. x 250mm
Eluent 20mM NaH2PO4 (pH=2.5, by 20mM H3PO4)
Flow rate 1.0mL/min, at 35C
Detector UV 210nm, 0.04AUFS.
Injection 10uL
Sample
  1. Oxalic Acid (0.02mg)
  2. Tartaric Acid (0.1mg)
  3. Formic Acid (0.2mg)
  4. Lactic Acid (0.55mg)
  5. Acetic Acid (0.5mg)
  6. Citric Acid (0.35mg)
  7. Fumaric Acid (0.003mg)
  8. Succinic Acid
  9. Acrylic Acid (0.014mg)
  10. Propionic Acid (1.2mg)
  11. Levulinic Acid (2.0mg) in 1mL Eluent
Category Acids

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Analysis of PTC-Amino Acid from Fingerprint (PDF file=52KB)

Column Wakosil-PTC, 4.0mm I.D. x 200mm
Eluent A) CH3CN/60mM CH3COONa(pH6.0)= 6/94(v/v)
B) CH3CN/60mM CH3COONa(pH6.0)= 60/40(v/v)
0-15min. B conc. 0-70%,
15-20min. B conc. 100%
Flow rate 1.0mL/min, at 40C
Detector UV 254nm
Sample
    PTC-
  1. Aspartic Acid
  2. Glutamic Acid
  3. Serine
  4. Glycine
  5. Histidine
  6. Arginine
  7. Threonine
  8. Alanine
  9. Proline
  10. Ammonia
  11. Tyrosine
  12. Valine
  13. Methionine
  14. Cystine
  15. Isoleucine
  16. Leucine
  17. Phenylalanine
  18. Tryptophan
  19. Lysine
Category Proteins, Peptides, Amino Acids

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Analysis of PTC-Amino Acid from Pig Insulin (PDF file=52KB)

Column Wakosil-PTC, 4.0mm I.D. x 200mm
Eluent A) CH3CN/60mM CH3COONa(pH6.0)= 6/94(v/v)
B) CH3CN/60mM CH3COONa(pH6.0)=60/40(v/v)
0-15min. B conc. 0-70%,
15-20min. B conc. 100%
Flow rate 1.0mL/min, at 40C
Detector UV 254nm
Sample
    PTC-
  1. Aspartic Acid
  2. Glutamic Acid
  3. Serine
  4. Glycine
  5. Histidine
  6. Arginine
  7. Threonine
  8. Alanine
  9. Proline
  10. Ammonia
  11. Tyrosine
  12. Valine
  13. Methionine
  14. Cystine
  15. Isoleucine
  16. Leucine
  17. Phenylalanine
  18. Lysine
Category Proteins, Peptides, Amino Acids

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Analysis of PTC-Amino Acid from Sweat (PDF file=52KB)

Column Wakosil-PTC, 4.0mm I.D. x 200mm
Eluent A) CH3CN/60mM CH3COONa(pH6.0)=6/94(v/v)
B) CH3CN/60/mM CH3COONa(pH6.0)=60/40(v/v)
0-15min. B conc. 0-70%,
15-20min. B conc. 100%
Flow rate 1.0mL/min, at 40C
Detector UV 254nm
Sample
    PTC-
  1. Aspartic Acid
  2. Glutamic Acid
  3. Serine
  4. Glycine
  5. Histidine
  6. Arginine
  7. Threonine
  8. Alanine
  9. Proline
  10. Ammonia
  11. Tyrosine
  12. Valine
  13. Methionine
  14. Cystine
  15. Isoleucine
  16. Leucine
  17. Phenylalanine
  18. Tryptophan
  19. Lysine
Category Proteins, Peptides, Amino Acids

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Analysis of PTC-Amino Acid standard (PDF file=54KB)

Column Wakosil-PTC, 4.0mm I.D. x 200mm
Eluent A) CH3CN/60mM CH3COONa(pH6.0)=6/94(v/v)
B) CH3CN/60mM CH3COONa(pH6.0)=60/40(v/v)
0-15min. B conc. 0-70%,
15-20min.　B conc. 100%
Flow rate 1.0mL/min, at 40C
Detector UV 254nm
Sample
    PTC-
  1. Aspartic Acid
  2. Glutamic Acid
  3. Serine
  4. Glycine
  5. Histidine
  6. Arginine
  7. Threonine
  8. Alanine
  9. Proline
  10. Ammonia
  11. Tyrosine
  12. Valine
  13. Methionine
  14. Cystine
  15. Isoleucine
  16. Leucine
  17. Phenylalanine
  18. Lysine
Category Proteins, Peptides, Amino Acids

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Analysis of PTH-Amino Acid Standard (PDF file=52KB)

Column Navi C22-5, 2.0mm I.D. x 250mm
Eluent A) CH3CN/50mM CH3COONH4 (pH5.0)=15/85(v/v)
add 1-Hexansulfonic Acid Sodium Salt 0.2%
B) 2-Propanol/H2O= 31/69(v/v)
1-16min. B conc. 0-100%, 16-20min. B conc. 100%, 20-20min. B conc. 0%
Flow rate 0.3mL/min, at 40C
Detector UV 269nm, 0.04AUFS.
Injection 25pmol
Sample
  1. Aspartic Acid
  2. Glutamic Acid
  3. Asparagine
  4. Serine
  5. Threonine
  6. Glycine
  7. Glutamine
  8. Histidine
  9. Alanine
  10. Arginine
  11. Tyrosine
  12. Δ-Threonine
  13. Proline
  14. Methionine
  15. Valine
  16. Tryptophan
  17. Lysine
  18. Phenylalanine
  19. Isoleucine
  20. Leucine
Category Proteins, Peptides, Amino Acids

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カザミノ酸中のアミノ酸分析 (PDF file=52KB)

Column Wakosil-PTC (4.0＊200mm)
Eluent A) PTC-AA buffer A
B) PTC-AA buffer B
0→15min. B=0→70%
15min.→ B=100%
Flow rate 1.0ml/min. at 40℃
Detector UV 254nm
Sample
  1. Asp (Aspartic Acid ; アスパラギン酸)
  2. Glu (Glutamic Acid ; グルタミン酸)
  3. Ser (Serine ; セリン)
  4. Gly (Glycine ; グリシン)
  5. His (Histidine ; ヒスチジン)
  6. Arg (Arginine ; アルギニン)
  7. Thr (Threonine ; トレオニン ; スレオニン)
  8. Ala (Alanine ; アラニン)
  9. Pro (Proline ; プロリン)
  10. NH3 (Ammonia ; アンモニア)
  11. Tyr (Tyrosine ; チロシン ; タイロシン)
  12. Val (Valine ; バリン)
  13. Met (Methionine ; メチオニン)
  14. Cys (Cysteine ; システイン)
  15. Ile (Isoleucine ; イソロイシン)
  16. Leu (Leucine ; ロイシン)
  17. Phe (Phenylalanine ; フェニルアラニン)
  18. Lys (Lysine ; リジン ; リシン)
    19. (いずれも、PTC標識化)
Category Acids

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タンパク質・ペプチドの分析 (PDF file=100KB)

Column Wakosil-II 3C18AR
(長さ5cm×内径4.6mm I.d.,和光純薬工業)(粒子径3μm)
Eluent 溶媒A：0.1%TFA水溶液
溶媒B：0.1%TFAアセトニトリル溶液
溶媒B 10%から42%へ12分のリニアグラジエント
Flow rate 1.0ml/min. at 35℃
Detector UV (220nm)
Sample
  1. Arg-バソプレシン
    (Arg-Vasopressin ; Arginine-Vasopressin ;
    アルギニン-バソプレシン)
  2. Met-エンケファリン
    (Met-Enkephalin ; Methionine-Enkephalin ;
    メチオニン-エンケファリン)
  3. オキシトシン (Oxytocin)
  4. ブラジキニン (Bradykinin)
  5. Leu-エンケファリン
    (Leu-Enkephalin ; Leucine-Enkephalin ;
    ロイシン-エンケファリン)
  6. アンギオテンシンI (Angiotensin I)
  7. リボヌクレアーゼ (Ribonuclease)
  8. インスリン (Insulin)
  9. チトクロームC (Cytochrome C)
  10. リゾチーム (Lysozyme)
Category Biomaterial aminoacid

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ドリンク剤中のタウリンの分析 (PDF file=48KB)

Column Wakosil-PTC (4.0＊200mm)
Eluent A) PTC-A buffer
B) PTC-B buffer
0→15min. B=0→70%
15→20min. B=100%
20→ B=0%
Flow rate 1.0ml/min. at 40℃
Detector UV 254nm,0.08AUFS.
Sample
  1. Taurine (タウリン) (PTC標識化)
Category Acids

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フタル酸、フタリド、安息香酸の分析 (PDF file=43KB)

Column Wakosil 5C18-200N (長さ15cm×内径4.6mm I.d., 和光純薬工業)(粒子径5μm)
Eluent アセトニトリル-リン酸 (pH 2.5) (30：70)
Flow rate 1.2ml/min. at 30℃
Detector UV (264nm)
Sample
  1. フタル酸 (Phthalic Acid)
  2. フタリド (Phthalide)
  3. 安息香酸 (Benzoic Acid)
Category Environmental Analysis, acids

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