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HPLCアプリケーションデータ
Analysis of Drugs

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• Analysis of Allantoin
• Analysis of Allantoin in Cream
• Analysis of Antipyrine
• Analysis of Bacitracin
• Analysis of Benzylpenicillinic Acid Potassium Salt
• Analysis of Betamethasone Valerate
• Analysis of Carazolol
• Analysis of Ceftiofur
• Analysis of Chlorhexidine Hydrochloride
• Analysis of Chlortetracyclin

Analysis of Allantoin (PDF file=49KB)

Column	Wakosil 5NH2, 4.6mm I.D. x 150mm
Eluent	CH3CN/H2O=70/30(v/v)
Flow rate	1.0mL/min, at R.T
Detector	UV 210nm, 0.16AUFS.
Injection	10uL, 100ug/mL (H2O)
Sample	Allantoin
Category	Drugs

Analysis of Allantoin in Cream (PDF file=49KB)

Column	Wakosil 5NH2, 4.6mm I.D. x 150mm
Eluent	CH3CN/H2O=70/30(v/v)
Flow rate	1.0mL/min, at R.T
Detector	UV 210nm, .16AUFS.
Injection	10uL, Cream 0.1g/mL(H2O), through 0.22um filter
Sample	Allantoin
Category	Drugs

Analysis of Antipyrine (PDF file=49KB)

Column	Wakosil 5C18 RS, 4.6mm I.D. x 150mm
Eluent	CH3OH/0.1% H3PO4=30/70(v/v)
Flow rate	1.0mL/min, at R.T.
Detector	UV 245nm, 0.16AUFS.
Injection	20uL
Sample	Antipyrine
Category	Drugs

Analysis of Bacitracin (PDF file=50KB)

Column	Wakosil-II5C18 RS, 4.6mm I.D. x 150mm
Eluent	20mM NaH2PO4, H3PO4 (pH3.0)/CH3CN=75/25(v/v)
Flow rate	1.0mL/min, at 40C
Detector	UV 210nm, 0.128AUFS.
Injection	10uL, 100ug/mL (H2O)
Sample	Bacitracin zinc salt
Category	Drugs

Analysis of Benzylpenicillinic Acid Potassium Salt (PDF file=50KB)

Column	Wakosil 5C18, 4.6mm I.D. x 250mm
Eluent	CH3CN/0.1% H3PO4=30/70(v/v)
Flow rate	1.0ml/min. at room temperature
Detector	UV 254nm, 0.10AUFS.
Injection	20ul, 2.0mg/ml (H2O)
Sample	benzylpenicillin ; benzylpenicillinic acid potassium salt
Category	Drugs

Analysis of Betamethasone Valerate (PDF file=39KB)

Column	Wakosil-II5C18 RS, 4.6mm I.D. x 10mm(precolumn)+4.6mm I.D. x 150mm
Eluent	CH3OH/H2O=70/10(v/v)
Flow rate	1.0mL/min, at R.T.
Detector	UV 254nm 0.064AUFS.
Injection	10uL
Sample	Betamethasone Valerate (0.1mg) Isoamyl Benzoate(I.S.) (0.1uL) in 2mL C2H5OH
Category	Drugs

Analysis of Carazolol (PDF file=49KB)

Column	Wakosil-II5C18 RS, 4.6mm I.D. x 150mm
Eluent	CH3CN/H2O/TFA=250/750/1(v/v/v)
Flow rate	1.0ml/min. at 35C
Detector	UV 254nm, 0.64AUFS.
Injection	10ul, 0.1mg/ml (CH3OH)
Sample	Carazolol; 1-(9H-carbazol-4-yloxy)-3-((1-methylethyl)amino)-2-propanol hydrochloride
Category	Drugs

Analysis of Ceftiofur (PDF file=50KB)

Column	Wakosil-II5C18 RS, 4.6mm I.D. x 150mm
Eluent	CH3CN/H2O/TFA=25/75/0.1(v/v/v)
Flow rate	1.0ml/min. at 35C
Detector	UV 254nm, 0.64AUFS.
Injection	10ul, 0.1mg/ml (CH3OH)
Sample	ceftiofur; (6R-(6a,7B(Z)))-7(((2-amino-4-thiazolyl)(methoxymino)acetyl)amino)- 3-(((2-furanylcarbonyl)-thio)methyl)-8-oxo-5-thia- 1-azabicyclo(4.2.0)oct-2-ene-2-carboxylic acid hydrochloride
Category	Drugs

Analysis of Chlorhexidine Hydrochloride (PDF file=49KB)

Column	Wakosil-II5C18 RS, 4.6mm I.D. x 150mm
Eluent	CH3OH/H2O/CH3COOH/SDS (Sodium Dodecyl Sulfate)=830/170/4/1.36(v/v/v/w)
Flow rate	1.0mL/min, at R.T.
Detector	UV 260nm, 0.128AUFS.
Injection	20uL, 30ug/mL
Sample	Chlorhexidine Hydrochloride
Category	Drugs

Analysis of Chlortetracyclin (PDF file=49KB)

Column	Wakosil-II5C18 RS, 4.6mm I.D. x 150mm
Eluent	CH3CN/10mM Oxalic Acid=20/80(v/v)
Flow rate	1.0ml/min. at 35C
Detector	UV 230nm, 0.32AUFS.
Injection	10ul, 1.0mg/ml (CH3OH)
Sample	chlortetracyclin ; chlortetracyclin hydrochloride
Category	Drugs