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HPLCアプリケーションデータ
Analysis of Foods/Food Additives

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• Analysis of Annatto
• Analysis of antibiotics in Honey
• Analysis of Beet Red
• Analysis of Carthamus Yellow
• Analysis of Catechin
• Analysis of Catechin and Caffein in Tea Bag
• Analysis of Cochineal
• Analysis of Corn
• Analysis of Ethyl α-D-Glucoside
• Analysis of Flubendazole

Analysis of Annatto (PDF file=50KB)

Column	Wakosil-II5C18 RS, 4.6mm I.D. x 150mm
Eluent	A) CH3CN/H2O=5/95(v/v), 0.05% TFA (Trifluoroacetic Acid) B) CH3CN/H2O=100/0(v/v), 0.05% TFA 0-20min. B conc. 50-100%, 20-25min. B conc. 100%
Flow rate	1.0mL/min, at 40C
Detector	UV Max. 310-800nm, 0.08AUFS.
Injection	10uL
Sample	Annatto
Category	Foods, additive to Foods

Analysis of antibiotics in Honey (PDF file=404KB)

Column	Navi C18-5, 4.6mm I.D.×150mm
Eluent	CH3CN/10mM Oxalic Acid=20/80(v/v)
Flow rate	1.0ml/min. 35℃
Detector	Tetravcyclines UV 370nm Chloramphenicol UV 280nm
Injection	100μl
Sample	OTC(Oxytetracycline) TC(Tetracycline) CTC(Chlortetracycline) CP(Chloramphenicol)
Category	Food

Analysis of Beet Red (PDF file=51KB)

Column	Wakosil-II5C18 RS, 4.6mm I.D. x 150mm
Eluent	A) CH3CN/H2O=5/95(v/v), 0.05% TFA (Trifluoroacetic Acid) B) CH3CN/H2O=100/0(v/v), 0.05% TFA 0-25min. B conc. 0%
Flow rate	1.0mL/min, at 40C
Detector	UV Max. 310-800nm, 0.04AUFS.
Injection	10uL, 10mg/mL (0.1% TFA)
Sample	Beet Red
Category	Foods, additive to Foods

Analysis of Carthamus Yellow (PDF file=51KB)

Column	Wakosil-II5C18 RS, 4.6mm I.D. x 150mm
Eluent	A) CH3CN/H2O=5/95(v/v), 0.05% TFA (Trifluoroacetic Acid) B) CH3CN/H2O=100/0(v/v), 0.05% TFA 0-20min. B conc. 0-50%, 20-25min. B conc. 50%
Flow rate	1.0mL/min, at 40C
Detector	UV Max. 310-800nm, 0.02AUFS.
Injection	10uL, 0.5mg/mL (0.1% TFA)
Sample	Carthamus Yellow
Category	Foods, additive to Foods

Analysis of Catechin (PDF file=50KB)

Column	Wakosil-II5C18 HG, 4.6mm I.D. x 250mm
Eluent	CH3CN/THF/0.2%H3PO4=14.3/1.7/84(v/v/v)
Flow rate	1.0mL/min, at 40C
Detector	UV 280nm, 0.64AUFS.
Injection	20uL
Sample	Epigallocatechin (EGC) Caffeine Epicatechin (EC) Epigallocatechin Gallate (EGCg) Epicatechin Gallate (Ecg)
Category	Foods

Analysis of Catechin and Caffein in Tea Bag (PDF file=283KB)

Column	Handy ODS, 4.6mm I.D.×250mm
Eluent	A）H2O/CH3COOH=100/1（v/v） B）CH3CN/CH3COOH=100/1（v/v） 0 -10min. B conc. 5%, 10 -40min. B conc. 5-20%, 40-50min. B conc. 20%, 50-55min. B conc. 50%, 55-70min. B conc. 5%
Flow rate	：1.0ml/min. at 50℃
Detector	：UV 280nm, 0.128AUFS
Injection	：10μl
Sample	Epigallocatechin(EGC) Catechin(C) Caffein Epigallocatechin Gallate(EGCG) Epicatechin(EC) Epicatechin Gallate(ECG)
Category	Food

Analysis of Cochineal (PDF file=49KB)

Column	Wakosil-II5C18 RS, 4.6mm I.D. x 150mm
Eluent	A) CH3CN/H2O=5/95(v/v), 0.05% TFA (Trifluoroacetic Acid) B) CH3CN/H2O=100/0(v/v), 0.05% TFA 0-20min. B conc. 0-50%, 20-25min. B conc. 50%
Flow rate	1.0mL/min, at 40C
Detector	UV Max. 310-800nm, 0.16AUFS.
Injection	10uL, 0.5mg/mL (0.1% TFA)
Sample	Cochineal
Category	Foods, additive to Foods

Analysis of Corn (PDF file=50KB)

Column	Wakosil-II5C18 RS, 4.6mm I.D. x 150mm
Eluent	A) CH3CN/H2O=5/95(v/v), 0.05% TFA (Trifluoroacetic Acid) B) CH3CN/H2O=100/0(v/v), 0.05% TFA 0-20min. B conc. 0-50%, 20-25min. B conc. 50%
Flow rate	1.0mL/min, at 40C
Detector	UV Max. 310-800nm, 0.08AUFS.
Injection	10uL, 2mg/mL (0.1% TFA)
Sample	Corn
Category	Foods, additive to Foods

Analysis of Ethyl α-D-Glucoside (PDF file=52KB)

Column	Wakosil 5NH2, 4.6mm I.D. x 250mm
Eluent	CH3CN/H2O=80/20(v/v)
Flow rate	1.0mL/min, at R.T.
Detector	RI, ATTEN＝8
Injection	10uL, 5mg/mL (H2O)
Sample	Ethyl α-D-Glucoside
Category	Foods

Analysis of Flubendazole (PDF file=48KB)

Column	Wakosil-II5C18 HG, 4.6mm I.D. x 150mm
Eluent	CH3CN/H2O=35/65(v/v)
Flow rate	0.7mL/min, at 40C
Detector	UV 313nm, 0.016AUFS.
Injection	5uL, 0.0mg/mL
Sample	Flubendazole
Category	Foods, additive to Foods