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HPLCアプリケーションデータ
Analysis of Foods/Food Additives

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• Analysis of Gardenia Yellow
• Analysis of Ivermectin
• Analysis of Lac Dye
• Analysis of Laccaic Acid
• Analysis of Lactoferrin
• Analysis of Metoprolol Tartrate
• Analysis of Monascus
• Analysis of Parabens
• Analysis of Parabens
• Analysis of Parabens

Analysis of Gardenia Yellow (PDF file=50KB)

Column	Wakosil-II5C18 RS, 4.6mm I.D. x 150mm
Eluent	A) CH3CN/H2O=5/95(v/v), 0.05% TFA (Trifluoroacetic Acid) B) CH3CN/H2O=100/0 (v/v), 0.05% TFA 0-20min. B conc. 0-100%, 20-25min. B conc. 100%
Flow rate	1.0mL/min, at 40C
Detector	UV Max. 310-800nm, 0.16AUFS.
Injection	10uL
Sample	Gardenia Yellow
Category	Foods, additive to Foods

Analysis of Ivermectin (PDF file=48KB)

Column	Wakosil-II5C18 HG, 4.6mm I.D. x 150mm
Eluent	CH3OH/H2O=95/5(v/v)
Flow rate	0.4mL/min, at 40C
Detector	UV 260nm, 0.064AUFS.
Injection	5uL, 0.1mg/mL
Sample	Ivermectine
Category	Foods, additive to Foods

Analysis of Lac Dye (PDF file=50KB)

Column	Wakosil-II5C18 RS, 4.6mm I.D. x 150mm
Eluent	A) CH3CN/H2O=5/95(v/v), 0.05% TFA (Trifluoroacetic Acid) B) CH3CN/H2O=100/0(v/v), 0.05% TFA 0-20min. B conc. 0-50%, 20-25min. B conc. 50%
Flow rate	1.0mL/min, at 40C
Detector	UV Max. 310-800nm, 0.08AUFS.
Injection	10uL
Sample	Lac Dye
Category	Foods, additive to Foods

Analysis of Laccaic Acid (PDF file=50KB)

Column	Navi C18-5, 4.6mm I.D. x 150mm
Eluent	A) 20mM KH2PO4, K2HPO4 (pH7.0) B) CH3OH/20mM KH2PO4, K2HPO4 (pH7.0)=50/50 0-20min. B conc. 0-100%
Flow rate	1.0mL/min, at 40C
Detector	Vis 400nm
Injection	5uL, 1.0mg/30mL (Eluent)
Sample	Laccaic Acid C Laccaic Acid B Laccaic Acid A
Category	Foods, additive to Foods

Analysis of Lactoferrin (PDF file=49KB)

Column	Wakosil 5C8, 4.6mm I.D. x 250mm
Eluent	A) CH3CN/0.5M NaCl=10/90(v/v), 0.03% TFA　　　 B) CH3CN/0.5M NaCl=50/50(v/v), 0.03% TFA 0-25min. B conc. 50-100%, 25-35min. B conc. 50%
Flow rate	1.0mL/min, at 40C
Detector	UV 280nm, 0.064AUFS.
Injection	20uL, 2.0mg/mL (0.5M NaCl)
Sample	Lactoferrin from Bovin Milk
Category	Foods

Analysis of Metoprolol Tartrate (PDF file=49KB)

Column	Wakosil 5C18 RS, 4.6mm I.D. x 150mm
Eluent	CH3CN/10mM H3PO4, 5mM 1-pentansultonic acid=20/80(v/v)
Flow rate	1.0mL/min, at 35C
Detector	UV 276nm, 0.02AUFS.
Injection	20uL, 20ug/mL (H2O)
Sample	Metoprolol Tartrate
Category	Foods

Analysis of Monascus (PDF file=52KB)

Column	Wakosil-II5C18 RS, 4.6mm I.D. x 150mm
Eluent	A) CH3CN/H2O=5/95(v/v), 0.05% TFA (Trifluoroactic Acid) 　　 B) CH3CN/H2O=100/0(v/v), 0.05% TFA 0-20min. B conc. 0-100%, 20-25min. B conc. 100%
Flow rate	1.0mL/min, at 40C
Detector	UV Max. 310-800nm,AUFS.
Injection	10uL, 0.5mg/mL (CH3OH)
Sample	Monascus
Category	Foods, additive to Foods

Analysis of Parabens (PDF file=50KB)

Column	Wakosil-II5C18 AR, 4.6mm I.D. x 150mm
Eluent	CH3OH/50mM NaH2PO4/50mM Na2HPO4=60/20/20(v/v/v) add. 2.5mM Centyltrimethylammonium chloride (pH5.7 add. H3PO4)
Flow rate	1.0ml/min. at 35C
Detector	UV 240nm 0.064AUFS.
Injection	5ul, 20ug/ml (CH3OH)
Sample	PHBA Methyl PHBA Ethyl PHBA iso-Propyl PHBA Propyl Benzoic Acid Salicylic Acid PHBA ; p-Hydroxy Benzoic Acid
Category	Foods

Analysis of Parabens (PDF file=50KB)

Column	Wakosil-II5C18 HG, 4.6mm I.D. x 150mm
Eluent	CH3OH/50mM NaH2PO4/50mM Na2HPO4=60/20/20(v/v/v) add. 2.5mM Centyltrimethylammonium chloride (pH5.7 add. H3PO4)
Flow rate	1.0ml/min. at 35C
Detector	UV 240nm 0.064AUFS.
Injection	5ul, 20ug/ml (CH3OH)
Sample	PHBA Methyl PHBA Ethyl PHBA iso-Propyl PHBA Propyl Benzoic Acid Salicylic Acid PHBA ; p-Hydroxy Benzoic Acid
Category	Foods

Analysis of Parabens (PDF file=50KB)

Column	Wakosil-II5C18 RS, 4.6mm I.D. x 150mm
Eluent	CH3OH/50mM NaH2PO4/50mM Na2HPO4=60/20/20(v/v/v) add. 2.5mM Centyltrimethylammonium chloride (pH5.7 add. H3PO4)
Flow rate	1.0ml/min. at 35C
Detector	UV 240nm 0.064AUFS.
Injection	5ul, 20ug/ml (CH3OH)
Sample	PHBA Methyl PHBA Ethyl PHBA iso-Propyl PHBA Propyl Benzoic Acid Salicylic Acid PHBA ; p-Hydroxy Benzoic Acid
Category	Foods