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HPLCアプリケーションデータ
Analysis of Peptides

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Analysis of Amyloid beta-Protein (PDF file=201KB)

Column Wakosil -Ⅱ 5C18 AR, 4.6mm I.D.×150mm
Eluent A）H2O 0.1%TFA（Trifluoroacetic Acid）
B）CH3CN 0.1%TFA（Trifluoroacetic Acid）
0 -25min. B conc.　20　-70%,
25 -30min. B conc.　70%
Flow rate 1.0ml/min.
Detector UV 220nm, 0.512AUFS
Injection 2μl
Sample
  1. Amyloid β-protein（1-40） 2mg/ml
Category Peptide

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Analysis of Amyloid β-protein (1-40) (PDF file=50KB)

Column Wakosil-II5C18 AR, 4.6mm I.D. x 150mm
Eluent A) H2O 0.1% TFA
B) CH3CN 0.1% TFA
0-25min. B conc. 20-70%,
25-30min. B conc. 70%
Flow rate 1.0mL/min, at 40C
Detector UV 220nm, 0.512AUFS.
Injection 2uL, 2mg/mL
Sample
  1. Amyloid β-protein (1-40)
Category Proteins, Peptides, Amino Acids

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Analysis of Endothelins (PDF file=51KB)

Column Wakosil-II5C18 AR, 4.6mm I.D. x 150mm
Eluent A) H2O 0.1 TFA
B) CH3CN 0.1% TFA
0-25min. B conc. 10-60%,
25-30min. B conc. 60%
Flow rate 1.0mL/min, at 40C
Detector UV 220nm, 0.256AUFS.
Injection 5uL, 10ug/mL (H2O)
Sample
  1. Endothelin-3
  2. Endothelin-1
Category Peptides

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Analysis of Enkephalins (PDF file=51KB)

Column Wakosil-II5C18 AR, 4.6mm I.D. x 150mm
Eluent A) H2O 0.1% TFA
B) CH3CN 0.1% TFA
0-25min. B conc. 10-60%,
25-30min.　B conc. 60%
Flow rate 1.0mL/min, at 40C
Detector UV 220nm, 0.256AUFS.
Injection 5uL
Sample
  1. Arg-Vasopressin
  2. Met-Enkephalin
  3. Leu-Enkephalin
  4. Substance P
Category Peptides

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Analysis of MW marker (PDF file=50KB)

Column Wakosil 5Diol-200, 8.0mm x 300mm
Eluent 0.1M NaH2PO4 (pH7.0) in 0.2M NaCl
Flow rate 1.0ml/min, at 40C
Detector UV 280nm, 0.002AUFS.
Injection 5ul
Sample
  1. Glutamate Dehydrogenase (MW 290,000)
  2. Lactate Dehydrogenase (MW 142,000)
  3. Enolase (MW 67,000)
  4. Adenylate Kinase (MW 32,000)
  5. Cytochrome C (MW 12,400)
Category Proteins, Peptides, Amino Acids

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Analysis of MW marker (PDF file=49KB)

Column Wakosil 5Diol-200, 8.0mm x 300mm
Eluent 0.1M NaH2PO4 (pH7.0) in 0.2M NaCl
Flow rate 1.0ml/min, at 40C
Detector UV 210nm, 0.32AUFS.
Injection 5ul
Sample
  1. Glutamate Dehydrogenase (MW 290,000)
  2. Lactate Dehydrogenase (MW 142,000)
  3. Enolase (MW 67,000)
  4. Adenylate Kinase (MW 32,000)
  5. Cytochrome C (MW 12,400)
Category Proteins, Peptides, Amino Acids

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Analysis of NBD-F-Tyrosine (PDF file=50KB)

Column Wakosil-II5C18 HG, 4.6mm I.D. x 250mm
Eluent A) 5mM NaH2PO4, Na2HPO4(pH6.8)
B) CH3CN
0-30min. B conc. 25-35%, 30-35min. B conc. 35%,
35-40min. B conc. 100%
Flow rate 1.0mL/min, at 40C
Detector Ex 488nm Em 540nm RANGE＝1, SENSE＝H, ATTEN＝1
Injection 20uL
Sample NBD-F- 1. o-Tyrosine 2. m-Tyrosine 3 p-Tyrosine
Category Proteins, Peptides, Amino Acids

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Analysis of PTC-Amino Acid from Fingerprint (PDF file=52KB)

Column Wakosil-PTC, 4.0mm I.D. x 200mm
Eluent A) CH3CN/60mM CH3COONa(pH6.0)=6/94(v/v)
B) CH3CN/60mM CH3COONa(pH6.0)=60/40(v/v)
0-15min. B conc. 0-70%, 15-20min. B conc. 100%
Flow rate 1.0mL/min, at 40C
Detector UV 254nm
Sample
    PTC-
  1. Aspartic Acid
  2. Glutamic Acid
  3. Serine
  4. Glycine
  5. Histidine
  6. Arginine
  7. Threonine
  8. Alanine
  9. Proline
  10. Ammonia
  11. Tyrosine
  12. Valine
  13. Methionine
  14. Cystine
  15. Isoleucine
  16. Leucine
  17. Phenylalanine
  18. Tryptophan
  19. Lysine
Category Proteins, Peptides, Amino Acids

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Analysis of PTC-Amino Acid from Pig Insulin (PDF file=52KB)

Column Wakosil-PTC, 4.0mm I.D. x 200mm
Eluent A) CH3CN/60mM CH3COONa(pH6.0)=6/94(v/v)
B) CH3CN/60mM CH3COONa(pH6.0)=60/40(v/v)
0-15min. B conc. 0-70%, 15-20min. B conc. 100%
Flow rate 1.0mL/min, at 40C
Detector UV 254nm
Sample
    PTC-
  1. Aspartic Acid
  2. Glutamic Acid
  3. Serine
  4. Glycine
  5. Histidine
  6. Arginine
  7. Threonine
  8. Alanine
  9. Proline
  10. Ammonia
  11. Tyrosine
  12. Valine
  13. Methionine
  14. Cystine
  15. Isoleucine
  16. Leucine
  17. Phenylalanine
  18. Lysine
Category Proteins, Peptides, Amino Acids

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Analysis of PTC-Amino Acid from Sweat (PDF file=52KB)

Column Wakosil-PTC, 4.0mm I.D. x 200mm
Eluent A) CH3CN/60mM CH3COONa(pH6.0)=6/94(v/v)
B) CH3CN/60/mM CH3COONa(pH6.0)=60/40(v/v)
0-15min. B conc. 0-70%, 15-20min. B conc. 100%
Flow rate 1.0mL/min, at 40C
Detector UV 254nm
Sample
    PTC-
  1. Aspartic Acid
  2. Glutamic Acid
  3. Serine
  4. Glycine
  5. Histidine
  6. Arginine
  7. Threonine
  8. Alanine
  9. Proline
  10. Ammonia
  11. Tyrosine
  12. Valine
  13. Methionine
  14. Cystine
  15. Isoleucine
  16. Leucine
  17. Phenylalanine
  18. Tryptophan
  19. Lysine
Category Proteins, Peptides, Amino Acids

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