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HPLCアプリケーションデータ
Analysis of Peptides

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• Analysis of PTC-Amino Acid standard
• Analysis of PTH-Amino Acid Standard

Analysis of PTC-Amino Acid standard (PDF file=54KB)

Column	Wakosil-PTC, 4.0mm I.D. x 200mm
Eluent	A) CH3CN/60mM CH3COONa(pH6.0)=6/94(v/v) B) CH3CN/60mM CH3COONa(pH6.0)=60/40(v/v) 0-15min. B conc. 0-70%, 15-20min. B conc. 100%
Flow rate	1.0mL/min, at 40C
Detector	UV 254nm
Sample	PTC- Aspartic Acid Glutamic Acid Serine Glycine Histidine Arginine Threonine Alanine Proline Ammonia Tyrosine Valine Methionine Cystine Isoleucine Leucine Phenylalanine Lysine
Category	Proteins, Peptides, Amino Acids

Analysis of PTH-Amino Acid Standard (PDF file=52KB)

Column	Navi C22-5, 2.0mm I.D. x 250mm
Eluent	A) CH3CN/50mM CH3COONH4 (pH5.0)=15/85(v/v) add 1 -Hexansulfonic Acid Sodium Salt 0.2% B) 2-Propanol/H2O=31/69(v/v) 1-16min. B conc. 0-100%, 16-20min. B conc. 100%, 20-20min. B conc. 0%
Flow rate	0.3mL/min, at 40C
Detector	UV 269nm, 0.04AUFS.
Injection	25pmol
Sample	Aspartic Acid Glutamic Acid Asparagine Serine Threonine Glycine Glutamine Histidine Alanine Arginine Tyrosine Δ-Threonine Proline Methionine Valine Tryptophan Lysine Phenylalanine Isoleucine Leucine
Category	Proteins, Peptides, Amino Acids