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試薬ホーム > HPLCアプリケーションデータ > Analysis of Biomaterials from Plants
HPLCアプリケーションデータ
Analysis of Biomaterials from Plants
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 Analysis of Carotene (PDF file=49KB)
| Column |
Navi C30-5, 4.6mm I.D. x 250mm |
 |
| Eluent |
CH3CN/MeOH/CHCl3=75/10/15 (v/v/v) |
| Flow rate |
2.0mL/min, at 35C |
| Detector |
UV 470nm, 0.16AUFS. |
| Injection |
5uL |
| Sample |
- α-Carotene
- β-Carotene each 0.05mg/mL (CHCl3)
|
| Category |
Sample of Plants |
Analysis of Carotenoid (PDF file=246KB)
| Column |
Navi C30-5, 4.6mm I.D.×250mm |
 |
| Eluent |
CH3CN/CH3OH/CHCl3=75/10/15（v/v/v） |
| Flow rate |
2.0ml/min. at 35℃ |
| Detector |
UV 470nm, 0.16AUFS |
| Injection |
10μl |
| Sample |
- α-Carotene
- β-Carotene
|
| Category |
Plant |
Analysis of Carotenoides (PDF file=49KB)
| Column |
Wakosil-DNPH, 4.6mm I.D. x 250mm |
 |
| Eluent |
CH3OH/TME/H2O=46/50/4(v/v/v) (TBME : t-Butyl Methyl Ether) |
| Flow rate |
0.7mL/min, at 35C |
| Detector |
Vis 451nm |
| Injection |
10uL |
| Sample |
- Zeaxanthin (2.0ug/mL)
- β-Criptoxanthin ( 2.0ug/mL)
- β-Carotene (3.483ug/mL)
|
| Category |
Plants |
Analysis of Catotenoides (PDF file=50KB)
| Column |
Wakosil-DNPH, 4.6mm I.D. x 250mm |
 |
| Eluent |
A) CH3OH/TBME/H2O=66/30/4 (v/v/v) (TBME : t-Butyl Methyl Ether)
B) CH3OH/TBME/H2O=46/50/4 (v/v/v) |
| Flow rate |
0.7mL/min, at 35C |
| Detector |
Vis 451nm, ATTN＝3 |
| Injection |
10uL |
| Sample |
- Zeaxanthin (2.0ug/mL)
- β-Criptoxanthin (2.0ug/mL)
- β-Carotene (3.483ug/mL)
|
| Category |
Plants |
Analysis of Dye containing Cedar Leaf (PDF file=261KB)
| Column |
Wakosil 5C18, 4.6mm I.D.×150mm |
 |
| Eluent |
A）CH3CN/CH3OH/0.1M TrisHCl （pH8.0）=75/12/4（v/v/v）
B）CH3OH/Ethylacetate=68/32（v/v）
0-10min. B conc. 0%,
10-30min. B conc. 0-100%,
30-40min. B conc. 100%, |
| Flow rate |
1.0ml/min. at R.T. |
| Detector |
UV 440nm, 0.016AUFS |
| Injection |
10μl |
| Sample |
- Neoxanthin
- Violaxanthin
- Lutein
- Zeaxanthin
- Chlorophyll a
- Chlorophyll b
- β-Carotene
|
| Category |
Plant |
Analysis of Sinigrin in Vegetable Extract (PDF file=280KB)
| Column |
Navi C18-5, 4.6mm I.D.×150mm |
 |
| Eluent |
A）（＊）Buffer B）CH3CN
0-10min. B conc. 15%,
10-15min. B conc. 50%,
15-40min. B conc. 15% |
| Flow rate |
1.0ml/min. at 35℃ |
| Detector |
UV 226nm |
| Injection |
10μl |
| Sample |
- Sinigrin
|
| Category |
Plant |
Analysis of Zeaxanthin and β-Carotene (PDF file=50KB)
| Column |
Wakosil-II5C18 HG, 4.6mm I.D. x 150mm |
 |
| Eluent |
A) CH3OH/H2O=90/10(v/v)
B) CH3OH/THF=95/5(v/v)
0-20min. B conc. 0-100%,
20-40min. B conc. 100%,
40-50min. B. conc. 0% |
| Flow rate |
1.5mL/min, at 40C |
| Detector |
Vis 450nm, 0.032AUFS. |
| Injection |
10uL |
| Sample |
- Zeaxanthin
- β-Carotene
|
| Category |
Sample of Plants |
アフラトキシン5種の一斉分析 (PDF file=88KB)
| Column |
Wakosil 5SIL (4.6＊250mm) |
 |
| Eluent |
Toluene/Ethyl Acetate/Formic Acid/CH3OH＝50/5/1/2(V/V/V/V) |
| Flow rate |
1.0ml/min. at R.T. |
| Detector |
λex=365nm,λem=425nm |
| Sample |
- Aflatoxin B1 (アフラトキシンB1)
- Aflatoxin B2 (アフラトキシンB2)
- Aflatoxin G1 (アフラトキシン)G1
- Aflatoxin G2 (アフラトキシンG2)
- Aflatoxin M1 (アフラトキシンM1)
|
| Category |
Foods
Plant |
クロロフィルa,bの分析 (PDF file=43KB)
| Column |
Wakosil 5SIL-120 (4.6＊150mm) |
 |
| Eluent |
n-Hexane/2-Propanol=98.5/1.5(V/V) |
| Flow rate |
1.0ml/min. at 0℃ |
| Detector |
0→6min. 430nm
6min.→ 460nm |
| Sample |
- Chlorophyll a (クロロフィルa)
- Chlorophyll b (クロロフィルb)
|
| Category |
Plants |
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