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HPLCアプリケーションデータ
Other Analysis

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• Analysis of 2-Phenoxyethanol and Methyl p-Hydroxy Benzoate
• Analysis of 3-Amino 4-Chloro Benzoic Acid
• Analysis of 4-Nitroquinoline 1-Oxide
• Analysis of Acid Red 33
• Analysis of Biuret
• Analysis of Diazirin
• Analysis of Flavon
• Analysis of Imidazole Acetic Acid
• Analysis of KNO₃ and KI
• Analysis of m-Phenylenediamine

Analysis of 2-Phenoxyethanol and Methyl p-Hydroxy Benzoate (PDF file=50KB)

Column	Hnady ODS, 4.6mm I.D. x 150mm
Eluent	CH3CN/0.1% H3PO4=20/80(v/v)
Flow rate	1.0mL/min, at 40C
Detector	UV 254nm, 0.06AUFS.
Injection	10uL
Sample	2-Phenoxyethanol (10uL/1000mL) Methyl p-Hydroxy Benzoate (10ug/mL) in CH3OH
Category	The Others

Analysis of 3-Amino 4-Chloro Benzoic Acid (PDF file=50KB)

Column	Wakosil-II5C18 RS, 4.6mm I.D. x 150mm
Eluent	CH3OH/20mM KH2PO4 (←pH3.0 H3PO4)=50/50(v/v)
Flow rate	8.0mL/min, at 50C
Detector	UV 235nm, 0.08AUFS.
Injection	20uL, 0.5mg/mL (Eluent)
Sample	3-Amino 4-Chloro Bezoic Acid
Category	The Others

Analysis of 4-Nitroquinoline 1-Oxide (PDF file=40KB)

Column	Wakosil-II5C18 HG, 4.6mm I.D. x 150mm
Eluent	CH3OH/50mM NaClO4 (pH3.5←50mm H3PO4)=30/70(v/v)
Flow rate	1.0mL/min, at 40C
Detector	UV 254nm 0.08AUFS.
Injection	10uL
Sample	4-Nitroquinoline 1-Oxide 1mM (DMSO/H2O＝1/9)
Category	The Others

Analysis of Acid Red 33 (PDF file=38KB)

Column	Wakosil-II5C18 AR, 4.6mm I.D. x 250mm
Eluent	A) CH3CN : 1/15M NaH2PO4=10/90(v/v) B) CH3CN 0-30min. B conc. 0-75%
Flow rate	1.0mL/min, at R.T.
Detector	Vis 500nm 0.64AUFS.
Injection	10uL
Sample	Acid Red 33 (R-227, Azofuchsin) 0.1mg/mL (H2O)
Category	The Others

Analysis of Biuret (PDF file=49KB)

Column	Wakosil 5NH2, 4.6mm I.D. x 250mm
Eluent	CH3CN/H2O/H3PO4=975/25/0.4(v/v/v)
Flow rate	1.0mL/min, at 35C
Detector	UV 200nm, 0.16AUFS.
Injection	10uL, 0.1mg/mL (Eluent)
Sample	Biuret
Category	The Others

Analysis of Diazirin (PDF file=50KB)

Column	Wakosil-II5C18 HG, 4.6mm I.D. x 150mm
Eluent	CH3CN/H2O=60/40(v/v)
Flow rate	1.0mL/min, at 40C
Detector	VIS. 300nm, 0.128AUFS.
Injection	10uL, 0.5mg/mL(CH3CN)
Sample	2-Methoxy-4-[3-(tritluoromethyl)-3H-diazirin-3-yl]benzoic Acid Methyl Ester
Category	The Others

Analysis of Flavon (PDF file=51KB)

Column	Wakosil-II5C18 RS, mm 4.6 x 250mm
Eluent	A) CH3CN/0.05M NaH2PO4 (pH2.3←H3PO4)=12/88(v/v) B) CH3CN/0.05M NaH2PO4 (pH2.3←H3PO4)=60/40(v/v)
Flow rate	1.0ml/min, at 40C
Detector	UV 325nm
Injection	10ul
Sample	Myricetin 0.60mg/100ml Morin 0.767mg/100ml Quercetin 0.633mg/100ml Kaempferol 0.713mg/100ml Flavon 0.71mg/100ml Karanjin 0.727mg/100ml 10ul inject.
Category	The Others

Analysis of Imidazole Acetic Acid (PDF file=51KB)

Column	Wakosil-II5C18 HG, 4.6mm I.D. x 150mm
Eluent	CH3CN/20mM KH2PO4, H3PO4 (pH3.3)=5/95(v/v)
Flow rate	1.0mL/min, at 35C
Detector	UV 210nm, 0.04AUFS.
Injection	10uL, 10ug/mK (H2O)
Sample	Imidazole-4-acetic Acid Hydrochloride
Category	The Others

Analysis of KNO₃ and KI (PDF file=40KB)

Column	Wakosil-II5C18 AR, 4.6mm I.D. x 150mm
Eluent	CH3CN/2mM TBAH/30mM NaPB=2/19/19(v/v/v)
Flow rate	0.8mL/min, at 40C
Detector	UV 225nm 0.16AUFS.
Injection	20uL
Sample	KNO3 (Potassium Nitrate) (34ug) KI (Potassium Iodide) (16.6ug) in 1mL H2O
Category	The Others

Analysis of m-Phenylenediamine (PDF file=42KB)

Column	Wakosil-II5C18 RS, 4.6mm I.D. x 150mm
Eluent	A) H2O B) CH3CN/H2O=50/50(v/v) 0-15min. B conc. 20-100%, 15-20min. B conc. 100%, 20-30min. B conc. 20%
Flow rate	1.0mL/min, at 35C
Detector	UV 254nm
Injection	20uL
Sample	m-Phenylenediamine 1mg/mL (H2O)
Category	The Others