試薬ホーム > HPLCアプリケーションデータ > Other Analysis

HPLCアプリケーションデータ
Other Analysis

Previous page　　Next page

• Analysis of NBD-F-Glutathiones
• Analysis of Oligo Deoxythymidine 12-18 mer
• Analysis of Oligo Deoxythymidine 12-18 mer
• Analysis of Phenols
• Analysis of p-Hydroxybenzoates
• Analysis of p-Hydroxybenzoates
• Analysis of p-Hydroxybenzoates and Salicylates
• Analysis of p-Hydroxybenzoates and Salicylates
• Analysis of Sodium Azulene Sultonate
• Analysis of Stellettamide A Trifluoroacetate

Analysis of NBD-F-Glutathiones (PDF file=50KB)

Column	Wakosil-II5C18 RS, 4.6mm I.D. x 150mm
Eluent	A) CH3CN/75mM H3PO4=16/84(v/v) B) CH3CN/CH3OH/50mM KH2PO4=21/39/40(v/v/v) 0-15min. B conc. 0%, 15-20min. B conc. 0-80%, 20-30min. B conc. 80-100% 30-40min. B conc. 0%
Flow rate	1.2mL/min, at 30C
Detector	Ex 470nm, Em 540nm, GAIN＝2, SENS＝1
Injection	20uL
Sample	Glutathione Sulfonic Acid Glutathione (Oxidized form) Glutathione (Reduced form)
Category	The Others

Analysis of Oligo Deoxythymidine 12-18 mer (PDF file=41KB)

Column	Wakosil-DNA, 20mm I.D. x 250mm
Eluent	A) H2O in 0.1M TEAB (PH 7.0) B) CH3CN/H2O=25/75(v/v) in 0.1M TEAB* (pH7.0) 0-25min. B conc. 45-50%
Flow rate	9.9mL/min, at R.T.
Detector	UV 270nm 0.08AUFS.
Injection	200uL
Sample	Oligo Deoxythymidine 12-18 mer 5unit/mL
Category	The Others

Analysis of Oligo Deoxythymidine 12-18 mer (PDF file=39KB)

Column	Wakosil-DNA, 4.6mm I.D. x 250mm
Eluent	A) H2O in 0.1M TEAB (pH7.0) B) CH3CN/H2O=50/50(v/v) in 1.0M TEAB* (pH7.0) 0-25 min. B conc. 20-24%
Flow rate	1mL/min, at 40C
Detector	UV 270nm 0.08AUFS.
Injection	10uL
Sample	Oligo Deoxythymidine 12-18 mer 5unit/mL
Category	The Others

Analysis of Phenols (PDF file=49KB)

Column	Wakosil-II5C18 HG, 4.6mm I.D. x 150mm
Eluent	CH3OH/0.1% H3PO4=10/90(v/v)
Flow rate	1.0mL/min, at 35C
Detector	UV 254nm, 0.016AUFS.
Injection	10uL, 40ug/mL (H2O)
Sample	Hydroquinone Catechol Phenol
Category	The Others

Analysis of Sodium Azulene Sultonate (PDF file=48KB)

Column	Wakosil-II5C18 RS, 4.6mm I.D. x 150mm
Eluent	A) 0.1M CH3COONH4 B) CH3OH B conc. 60%
Flow rate	1.0mL/min, at 35C
Detector	UV 290nm, 0.128AUFS.
Injection	5uL, 0.01% (H2O)
Sample	Sodium Azulene Sulfonate
Category	The Others

Analysis of Stellettamide A Trifluoroacetate (PDF file=39KB)

Column	Wakosil-II5C18 AR, 4.6mm I.D. x 150mm
Eluent	2-Propanol/50mM NaClO4, H3PO4(pH3.5)=35/65(v/v)
Flow rate	1.0mL/min, at 50C
Detector	UV 254nm
Injection	2uL
Sample	Stellettamide A Trifluoroacetate 5mg/mL (CH3OH)
Category	The Others