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HPLCアプリケーションデータ
Analysis of Vitamins

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Analysis of Fat-soluble Vitamins (PDF file=280KB)

Column Navi C30-5, 4.6mm I.D.×250mm 　
Eluent CH3OH/CH3CN=25/75（v/v）
Flow rate 1.0ml/min. at 40℃
Detector UV 280nm, 0.16AUFS
Injection 10μl
Sample
  1. Vitamin K3
  2. Vitamin A
  3. Vitamin D2
  4. Vitamin D3
  5. Vitamin E
  6. Vitamin E Acetate
  7. Vitamin K1
Category Vitamin

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Analysis of Oil Soluble Vitamins (PDF file=51KB)

Column Navi C18-5, 4.6mm I.D. x 250mm 　
Eluent CH3OH/CH3CN=25/75(v/v)
Flow rate 1.0mL/min, at 40C
Detector UV 280nm, 0.16AUFS.
Injection 10uL
Sample
  1. Vitamin K3 (0.03mg)
  2. Vitamin A (0.03mg)
  3. Vitamin D2 (0.03mg)
  4. Vitamin D3 (0.03mg)
  5. Vitamin E (0.3mg)
  6. Vitamin E Acetate (0.4mg)
  7. Vitamin K1 (0.1mg) in 1mL CH3CN
Category Vitamins

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Analysis of Tocopherol (PDF file=49KB)

Column Navi C18-5, 4.6mm I.D. x 250mm 　
Eluent CH3CN
Flow rate 1.0mL/min, at 35C
Detector UV 295nm, 0.064AUFS.
Injection 10uL, 0.1mg/mL(IPA)
Sample
  1. d-δ-Tocopherol
  2. d-γ-Tocopherol
  3. d-β-Tocopherol
  4. d-α-Tocopherol
Category Vitamins

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Analysis of Tocopherol (PDF file=49KB)

Column Navi C30-5, 4.6mm I.D. x 250mm 　
Eluent CH3CN
Flow rate 1.0mL/min, at 35C
Detector UV 295nm, 0.064AUFS.
Injection 10uL, 0.1mg/mL(IPA)
Sample
  1. d-δ-Tocopherol
  2. d-γ-Tocopherol
  3. d-β-Tocopherol
  4. d-α-Tocopherol
Category Vitamins

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Analysis of Vitamin C of Coffee Beverage (PDF file=221KB)

Column Navi C18-5, 4.6mm I.D.×150mm 　
Eluent 10mM H3PO4
Flow rate 1.0ml/min. at 35℃
Detector UV 270nm
Injection 10μl
Sample
  1. Vitamin C
Category Vitamin

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Analysis of Vitamin D3 and Metabolites (PDF file=49KB)

Column Wakosil 5SIL-120, 4.6mm I.D. x 250mm 　
Eluent 2-Propanol/n-Hexan=20/80(v/v)
Flow rate 1.0mL/min, at 40C
Detector UV 265nm, 0.128AUFS.
Injection 5uL, 0.03mg/mL (EtOH)
Sample
  1. Vitamin D3
  2. 25(OH) D3 (Calcifediol)
  3. 24,25(OH)3D3 (24(R),25-Dihydroxy Vitamin D3)
  4. 1,25(OH)3 D3 (Calcitriol)
Category Vitamins

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Analysis of Vitamin E Homolog (PDF file=278KB)

Column Navi C30-5, 4.6mm I.D.×250mm 　
Eluent CH3CN=100%
Flow rate 1.0ml/min. at 35℃
Detector UV 295nm, 0.064AUFS
Injection 10μl
Sample
  1. d-δ-Tocopherol
  2. d-γ-Tocopherol
  3. d-β-Tocopherol
  4. d-α-Tocopherol
Category Vitamin

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Analysis of Vitamine D3 and Metabolites (PDF file=49KB)

Column Navi C18-5, 4.6mm I.D. x 250mm
Eluent CH3CN
Flow rate 1.0mL/min, at 40C
Detector UV 265nm, 0.128AUFS.
Injection 5uL, 0.03mg/mL (EtOH)
Sample
  1. 1,25(OH)3 D3 (Calcitriol)
  2. 24, 25(OH)3 D3 (24(R), 25-Dihydroxy Vitamin D3)
  3. 25(OH) D3 (Calcifediol)
  4. Vitamin D3
Category Vitamins

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Analysis of Water soluble Vitamins (PDF file=49KB)

Column Wakosil-II5C18 RS, 4.6 x 150mm
Eluent CH3CN/0.1%H3PO4=10/90(v/v), 10nM Sodium
Flow rate 1.0ml/min, at 40C
Detector UV 270nm, 0.64AUFS.
Injection 10ul
Sample
  1. Nicotinamide 200ug/ml (H2O)
  2. VB6 100ug/ml (H2O)
  3. Riboflavin Sodium Phosphate 100ug/ml (H2O)
Category Vitamins

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Analysis of Water-soluble Vitamins (PDF file=335KB)

Column Navi C18-5, 4.6mm I.D.×250mm
Eluent CH3CN/0.1%H3PO4, 5mM SHS*）=10/90（v/v）
Flow rate 1.0ml/min. at 40℃
Detector UV 210nm, 0.16AUFS
Injection 5μl
Sample
  1. L（＋）-Ascorbic acid（V.C）
  2. Nicotinic acid
  3. Nicotinamide
  4. Pyridoxine・HCl（V.B1）
  5. Caffein
  6. Thiamine・HCl（V.B1）
  7. d-Biotin（B.H）
  8. Riboflavin（V.B2）
Category Vitamin

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